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    • View All Programs
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Transformation & Gene Regulation VCE Biology

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Program Details

  • VCE
  • 24 Students
  • 1 Day
  • Onsite
  • Online N/A

About

Curriculum Focus

  • Unit 3 (AoS1): What is the role of nucleic acids and proteins in maintaining life?
  • Unit 4 (AoS3): How is scientific inquiry used to investigate cellular processes and/or biological change?

Technical and Personal Skills

Scientific inquiry, key science skills, problem solving, collaboration, communication

 

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Students will get hands-on experience in modern molecular biology techniques. They will work with a plasmid containing the insulin gene fused to the green fluorescent protein (GFP), under the control of the arabinose operon.

This program enables students to investigate the process of bacterial transformation as they introduce the GFP containing plasmid into E. coli and select for its presence using an antibiotic. They will also investigate gene regulation in bacteria as they induce expression of GFP by way of the inclusion of arabinose in the growth medium and fluorescence by excitation under UV light.

Students will set up and run a controlled scientific experiment that clearly demonstrates the relationship between independent and dependent variables.

There is time to complete one elective activity. Please see program variation for options.

Students will

  • Undertake laboratory work to perform a genetic transformation
  • Apply an understanding of gene regulation to control expression of a fluorescent protein
  • Analyse and interpret experimental results
  • Tour a modern research laboratory
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Program Variation

Select 1 of:

  • Fiona Elsey Cancer Research Institute
  • Restriction digest followed by electrophoresis
  • Column chromatography of expressed protein

The Ballarat Tech School is a Victorian State Government initiative.

All Enquiries

ballarattechschool@federation.edu.au
03 5327 8080

Campus Location

MB John Building, Level 1 FedUni SMB Campus,
136 Albert St, Ballarat Central VIC 3350

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Victoria state government education and training
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The Ballarat Tech School acknowledges the Traditional Owners of the land and waters where our campus is located, the Wadawurrung People. We pay our respects to their Elders past, present and emerging and extend this to all Aboriginal and Torres Strait Islander First Nations Peoples.

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